South Lake Union Campus
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• The role of autoantibodies to GAD65 (GAD65Ab) in the pathogenesis of autoimmune diabetes. In marked contrast to the traditional view, our recent studies and preliminary results suggest that GAD65Ab are common in healthy individuals, but masked by epitope-specific anti-idiotypic antibodies.• Specific depletion of GAD65-specific B lymphocytes in NOD mice. Injection with GAD65Ab-specific anti-Id-8E6G4 reduced the number of GAD65-specific B plasma cells by 85%. Investigation of GAD65-specific T cell responses in NOD mice injected with the above antibody are currently underway.• GAD65Ab in neurological disorders. GAD65Ab may have a pathogenic role in the disease process in patients with neurological disorders, including Stiff Person Syndrome (SPS), cerebellar ataxia (CA) and autoimmune epilepsy. GAD65Ab in these disorders may cause a deficit in the major inhibitory neurotransmitter GABA, possibly causing clinical symptoms such as muscle spasms and stiffness. In collaboration with Drs. Honnorat and Manto in France and Belgium, we investigate the role of GAD65Ab in the pathogenesis of neurological disorders, using in vitro and in vivo approaches.